Fig. 5: Comparison between non-PDA, pre-ligation, post-ligation at 4–8 h, and post-ligation at 24–48 h.

Changes in LVGLS, RVFWSL, and LASr across four time points: non-PDA group, pre-ligation, 4–8 h post-ligation, and 24–48 h post-ligation. Error bars represent mean ± SD. Statistical annotations: *p < 0.05 vs. non-PDA; †p < 0.05 vs. pre-ligation; ‡p < 0.05 vs. 4–8 h post-ligation. |LVGLS| absolute value of left ventricular global longitudinal strain (average of A4C, A2C, and A3C), |RVFWSL| absolute value of right ventricular free wall longitudinal strain, LASr left atrial reservoir strain, PDA(-) non-PDA, PDA patent ductus arteriosus.