Fig. 5: Ureaplasma infection results in oxidative stress. | Pediatric Research

Fig. 5: Ureaplasma infection results in oxidative stress.

From: Ureaplasma parvum and Ureaplasma urealyticum induce distinct types of inflammation in neonates and human epithelial cell models

Fig. 5

Alveolar epithelial cells were activated with indicated U. urealyticum (UU) or U. parvum (UP) isolates at a MOI 10 or 100. UP#1 = U. parvum clinical isolate 2990, UP#2 = U. parvum ATCC 700970, UP#3 = U. parvum ATCC 27815; UU#1 = U. urealyticum ATCC 27618, UU#2 = U. urealyticum ATCC 27817, UU#3 = U. urealyticum clinical isolate 2983. a To assess ROS generation, cells were stained with DCFDA, and after 3 h culture with Ureaplasma bacteria at MOI 10, ROS was detected by fluorescence spectroscopy with excitation/emission at 485/535 nm. b Cells were cultured with Ureaplasma bacteria at MOI 100, and after 24 h the activity of the antioxidant GSH-Px was determined using a commercial kit, and c the induction of apoptosis was determined by staining cells with Annexin-V antibody. Cells were analyzed by fluorescence microscopy and Annexin-V positive events were quantified using ImageJ. Representative results of two independent experiments. (One-way ANOVA followed by Dunnett’s multiple comparison test).

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