Table 2 Representative therapeutic RNAi agents
From: Engineered mitochondria in diseases: mechanisms, strategies, and applications
Tools | Target | Mechanism of action | Example | Ref |
|---|---|---|---|---|
siRNA | Specific mitochondrial mRNAs | Binding to complementary sequences in mitochondrial mRNAs, leading to their degradation and preventing translation. | siRNA targeting ND1, COXI, COXII, and COXIII in HEK293T cells | |
shRNA | Specific mitochondrial mRNAs | Processed into siRNA-like molecules that bind to and promote the degradation of specific mitochondrial mRNAs. | shRNA targeting TFAM in SW480 and Caco-2 cells | |
miRNA | Specific mitochondrial mRNAs | Binding to target sites on mitochondrial mRNAs, leading to translational repression or degradation | miRNA-2392 regulating oxidative phosphorylation and glycolysis in tongue squamous cell carcinoma | |
lncRNA | Mitochondrial gene expression regulators | Interacting with mitochondrial mRNAs or proteins involved in mitochondrial transcription and translation | lncRNA targeting cytochrome B ameliorated mtDNA damage in retinal endothelial cells |
