Fig. 1: Overview of differentially methylated CpG sites (DMC) in the blood of newborn guinea pigs exposed to antenatal betamethasone.

A Scatterplot of individual CpG sites that were significantly differentially methylated in ACS-exposed animals as compared to unexposed controls. 956 sites were hypermethylated, 721 sites were hypomethylated. >10× reads; ≥5% methylation difference; FDR ≤ 0.05 (B) DMCs were localized to various genomic features, based on distance from transcription start sites. Promoter: ≤1000 bp, Intergenic: ≥1000 bp, Intragenic: within gene body, including introns and exons (C) DMCs were annotated to known genes. 171 genes were identified in total, of which 108 genes were hypermethylated and 63 genes were hypomethylated. D The top 20 terms from the gene set enrichment analysis are represented from most to least significant. All differentially methylated genes discovered in the PND1 Blood (171 genes) were used in the analysis.