Fig. 2: β4OE is significantly reduced DSD in female mice regardless of estrous stage.

A Estrous stage breakdown of DSD of neurons from female CN and β4OE mice. Spine density was significantly different based on estrous stage (F = 6.450, DF = 2, p = 0.002). Importantly, note both genotypes are represented in metestrus/diestrus, not proestrus, estrus. Data points are from individual neurons. Error bars = SD. ANOVA (α = 0.05). B β4OE significantly reduced DSD in female mice in metestrus/diestrus (F = 6.190, DF = 1, p = 0.017). Data points are from individual neurons. Error bars = SD. ANOVA (α = 0.05). C Within mouse internal control comparison. DSD of β4OE+ neurons was significantly lower than DSD of β4OE− internal control neurons in three β4OE mice overall (F = 103.646, DF = 1, p < 0.001). There was a significant interaction between the estrous stage and condition (β4OE+ vs β4OE−) (F = 8.105, DF = 1, p = 0.006), however the main effect of the estrous stage was not significant (F = 0.985, DF = 1, p = 0.324). Mice were in different estrous stages (L-R): Ms10-87 was in Estrus (F = 67.688, DF = 1, p < 0.001), and Ms5-10 (F = 9.502, DF = 1, p = 0.004) and Ms16-169 (F = 126.518, DF = 1, p < 0.001) were in metestrus/diestrus on day of sacrifice. Data points from individual neurons. Error bars = SD. ANOVA (α = 0.05). D DSD of β4OE− internal control neurons in the three β4OE mice (in C) did not significantly differ based on estrous stage (F = 2.108, DF = 1, p = 0.154). Data points from individual neurons. Error bars = SD. ANOVA (α = 0.05).