Fig. 3: β4OE selectively reduced mean density of spines with small volumes and decreased the density dendritic protrusions in female mice. | Translational Psychiatry

Fig. 3: β4OE selectively reduced mean density of spines with small volumes and decreased the density dendritic protrusions in female mice.

From: Impacts of CACNB4 overexpression on dendritic spine density in both sexes and relevance to schizophrenia

Fig. 3

A Mean DSD of small <0.1 μm3 objects was significantly reduced in neurons from female β4OE mice, relative to CN (F = 5.276, DF = 1, p = 0.035). The mean DSD of larger objects with volumes > 0.1 μm3 did not significantly differ based on genotype in female mice. Error bars = SEM. ANOVA (α = 0.05). B Focusing exclusively on female mice in metestrus/diestrus, β4OE significantly decreased the mean DSD of small objects of 0.1 μm3–0.2 μm3 volume (F = 6.748, DF = 1, p = 0.032). Error bars = SEM. ANOVA (α = 0.05). C β4OE significantly reduced mean density of short stubby (F = 33.939, DF = 1, p < 0.001), long stubby (F = 7.043, DF = 1, p = 0.009), short mushroom (F = 4.342, DF = 1, p = 0.040) and long mushroom (F = 30.354, DF = 1, p < 0.001) spines in female mice (all estrous stages represented) using ANOVA (α = 0.05). Collectively, spines in these four categories made up 95.19% of all protrusions observed in female mice. Error bars = SEM. D Focusing exclusively on female mice in metestrus/diestrus, β4OE significantly decreased mean density of short mushroom spines (F = 20.160, DF = 1, p < 0.001). Error bars = SEM.

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