Fig. 3: Differentially expressed genes (DEGs) in Clcn4 KD mNPCs. | Translational Psychiatry

Fig. 3: Differentially expressed genes (DEGs) in Clcn4 KD mNPCs.

From: Developmental deficits, synapse and dendritic abnormalities in a Clcn4 KO autism mice model: endophenotypic target for ASD

Fig. 3

a To explore the effects of Clcn4 deficiency, RNA sequencing was performed on mNPCs. Hierarchical clustering of three WT and KD lines based on differentially expressed RNA transcripts (2.0 FC, nbinomWaldTest test p < 0.01) from DESeq2. The expression level of each gene in a single sample is depicted according to the color scale. Volcano plot of DEGs in KD. The Y-axis displays the -log10 p-value for each gene, while the X-axis displays the log2 fold change for that gene relative to KD. b–f Top 10 enriched GO terms for DEGs. b GO term enrichment analysis for downregulated genes in WT and KD, (c) upregulated genes. d Hierarchical clustering of three KD and KD+Ris lines based on differentially expressed RNA transcripts (2.0 FC, nbinomWaldTest test p < 0.01) from DESeq2. The expression level of each gene in a single sample is depicted according to the color scale. Volcano plot of DEGs in KD. The Y-axis displays the -log10 p-value for each gene, while the X-axis displays the log2 fold change for that gene relative to KD. e GO term enrichment analysis for downregulated genes in KD and KD+Ris, f upregulated genes. Node size represents gen ratio; node color represents -log10 p-value. GO, gene ontology.

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