Fig. 2: GABAAR α1 subunit is a substrate of PPT1 enzyme.
From: GABAAR-PPT1 palmitoylation homeostasis controls synaptic transmission and circuitry oscillation
Representative immunoblot (A) and quantification (B) of GABAAR α1 subunits levels on the cellular membrane in WT and PPT1-KI mice at indicated ages. N = 3 for each group, two-way ANOVA, *P < 0.05. C Quantitative PCR showing developmental changes in the mRNA expression of GABAAR α1 subunit at the indicated ages. N = 3, two-way ANOVA, *P < 0.05. Representative immunoblotting (D) and quantification (E) of GABAAR α1 subunit levels on the cellular membrane in WT, PPT1-KI, and PPT1-KI mice treated with BuHA. N = 3 for each group, one-way ANOVA; *P < 0.05, **P < 0.01. F, G Increased levels of the palmitoylated GABAAR α1 subunit in cultured PPT1-KI neurons, which were recovered by incubation with BuHA. Representative images (H) and colocalization analysis (I) of GABAAR α1 subunit and PPT1 enzyme in cultured hippocampal neurons from WT mice. Fluorescence intensity was measured using the red line across the cell body. N = 8 cells. +HA, with hydroxylamine; N = 3 for each group, one-way ANOVA, *P < 0.05; **P < 0.01. Data are represented as mean ± SEM.
