Fig. 3: Identification of the binding sites between GABAAR α1 subunit and PPT1. | Translational Psychiatry

Fig. 3: Identification of the binding sites between GABAAR α1 subunit and PPT1.

From: GABAAR-PPT1 palmitoylation homeostasis controls synaptic transmission and circuitry oscillation

Fig. 3

(A) Interaction between PPT1 and GABAAR α1 subunit verified by in vitro CO-IP using mouse hippocampal tissue. IB: immunoblotting. (B) Bioinformatic prediction of palmitoylation site (Cys). (C, D) Identification of palmitoylation sites within GABAAR α1 subunit. Mutation of Cys-260 residues to alanine reduce palmitoylation level of GABAAR α1 in 293 T cell line. N = 3, one-way ANOVA, *P < 0.05. (E-H) Representative blots and quantification of immunoprecipitation show that mutations of Cys-165 and Cys-179 (E, F), but not Cys-260 or Cys-319 (G, H), to alanine block GABAAR α1 subunit binding to PPT1 in 293 T cell line. N = 3, one-way ANOVA, **P < 0.01.

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