Table 1 Nuclease expression vector for Gabra1 point mutation knock-in.
From: GABAAR-PPT1 palmitoylation homeostasis controls synaptic transmission and circuitry oscillation
Vector name | Sequence |
|---|---|
gRNA-A1 (matches forward strand of gene) | CATTGGGCATTCAGCTCTCA-CGG |
gRNA-A2 (matches reverse strand of gene) | CGAATTTTAGTGGGCAGGCA-TGG |
Donor oligo | TGCACTTTACTTCCATCTCTTCTGTTTGCTAAGGTTAATACCATCCTACTCTAATTATCAATATCTAA TCTATAGAGCTTTTATTTGTATTCTGTGTTCCCTTAATAAGGTTGACCGTGAGAGCGGAAAGCCCA ATGCACTTGGAAGACTTCCCTATGGATGCCCATGCCAGCCCTCTAAAATTCGGAAGCTGTAAGTAAATTTACTTCAAACTATCATTATTTTTTTGTCTACTAGTTTATTTATTTATTCACTTTACATCCAGATTGC |
TATTCCCCTCCCAGTCTCCCCTCCTCTA | |
CrRNA-A1 | 5′-CAUUGGGCAUUCAGCUCUCAGUUUUAGAGCUAUGCUGUUUUG-3′ |
CrRNA-A2 | 5′-CGAAUUUUAGUGGGCAGGCAGUUUUAGAGCUAUGCUGUUUUG-3′ |
TracrRNA | 5′-AAACAGCAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCU-3′ |
