Fig. 3: Effects of inhibition of BDNF downstream mediators on N2O-induced addiction, the expression changes of BDNF and its downstream mediators after N2O exposure. | Translational Psychiatry

Fig. 3: Effects of inhibition of BDNF downstream mediators on N2O-induced addiction, the expression changes of BDNF and its downstream mediators after N2O exposure.

From: Nitrous oxide exerts rewarding effect via regulating D1 receptor and BDNF pathway in ventral tegmental area-nucleus accumbens dopamine circuit

Fig. 3

A Effect of ANA-12 on N2O-induced CPP behaviors in mice (n = 9 / group). B Effect of SCH 772984 on N2O-induced CPP behaviors in mice (n = 9 / group). C Effect of KG 501 on N2O-induced CPP behaviors in mice (n = 9 / group). D Effect of N2O exposure on the expression of VTA’s BDNF mRNA (n = 3 / group). E Effect of N2O exposure on the expression of VTA’s BDNF protein (n = 3 / group). F Effect of N2O exposure on the expression of NAc’s p-Trkb protein (n = 3 / group). G Effect of N2O exposure on the expression of NAc’s p-ERK1/2 protein (n = 3 / group). H Effect of N2O exposure on the expression of NAc’s p-CREB protein (n = 3 / group). I, J Effect of N2O exposure on NeuN/p-ERK1/2 positive neurons of the NAc (n = 3 / group). Scale bar: 50 μm. K, L Effect of N2O exposure on NeuN/c-Fos positive neurons of the NAc (n = 3 / group). Scale bar: 50 μm. All data are means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.

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