Fig. 4: Dose-response effects induced by alprazolam: identification of the optimal dose and treatment duration that avoids sedative effects while potentially retaining anxiolytic properties. | Translational Psychiatry

Fig. 4: Dose-response effects induced by alprazolam: identification of the optimal dose and treatment duration that avoids sedative effects while potentially retaining anxiolytic properties.

From: First evidence of an anxiety-like behavior and its pharmacological modulation in a molluscan model organism, Lymnaea stagnalis

Fig. 4

A The timeline of the experiment is presented above the data. Snails were exposed to alprazolam 0.1 μM (B), 1 μM (C), or 10 μM (D) for 15, 30, and 60 min, and the feeding behavior was tested 3 h later in lettuce slurry. Control snails (CTRL) were not treated. We found that alprazolam 1 μM and 10 μM treatment led to a significant reduction in feeding behavior across all treatment durations (15, 30, and 60 min), with the sole exception of 0.1 μM for 15 min. N = 13-14. Data are represented as means ± SEM. Data shown in Figs. 4B and D were analyzed using the Kruskal-Wallis H test, followed by Dunn’s post-hoc, whereas data shown in Fig. 4C were analyzed with one-way ANOVA followed by Tukey post hoc. ****p < 0.0001, ** p < 0.01, ns = not significant as p > 0.05.

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