Fig. 5: Exposure to alprazolam (0.1 μM for 15 min) prevents the anxiety-like behaviors induced in snails by fish water.

A Study design: 4 distinct experimental conditions were used in this study: animals treated with PW for 15 min and tested in PW 3 h later (beige bars); animals treated with alprazolam 0.1 μM for 15 min and tested in PW 3 h later (diagonal beige bars); animals treated with PW for 15 min and tested in FW 3 h later (green bars), and animals treated with aprazolam 0.1 μM for 15 min and tested in FW 3 h later (diagonal green bars). B Aerial Respiration: alprazolam prevented the FW-induced increase in breathing time. C Righting Behavior: alprazolam prevented the FW-induced reduction in righting time. D Escape Behavior: alprazolam prevented the FW-induced reduction in escape behavior. Data are represented as means ± SEM and analyzed with the Kruskal-Wallis H test, followed by Dunn’s post-hoc. ****p < 0.0001, ns = not significant as p > 0.05.