Fig. 3: 15q11.2 deletion decreases neurite complexity and length in both glutamatergic and GABAergic neurons. | Translational Psychiatry

Fig. 3: 15q11.2 deletion decreases neurite complexity and length in both glutamatergic and GABAergic neurons.

From: Altered development and network connectivity in a human neuronal model of 15q11.2 deletion-related neurodevelopmental disorders

Fig. 3

A, B 3D reconstructions of 25X oil immersion confocal images of pAAV9-CaMKIIa-hChR2(H134R)-EYFP (CAMKIIα-YFP) and pAAV9-mDlx-GFP-Fishell-1 (DLX-GFP) expressing representative neurons with corresponding Sholl intersections in control and 15q11.2 deletion neurons at DIC 141. Panels on the left in A and B demonstrate reconstruction of anti-GFP immunostaining (green) with Sholl intersection overlay (multicolored spheres). Panels on the right demonstrate inverted images with Sholl intersection and neurite skeleton only. Scale bar 50 µm. C Sholl analysis of CAMKIIα-YFP+ neurites from N = 33 control cells from 3 lines and N = 33 deletion cells from 3 lines ad DIC 141. Two-way ANOVA P < 0.0001. D Sholl analysis of DLX-GFP+ neurites from N = 41 control cells from 4 donor lines and N = 30 deletion cells from 3 donor lines at DIC 141. Two-way ANOVA P < 0.0001. Tukey’s multiple comparisons test * = P < 0.05, ** = P < 0.01, *** = P < 0.001, **** = P < 0.0001. E Scholl intersections by line at 80 µm from data in C and D. Columns represent mean per line and symbols are individual neurons. Nested one-way ANOVA P < 0.0001. F Average total neurite length per cell is decreased in deletion compared to control donor lines. Column is the mean per line and each symbol is total neurite length for a single neuron. Nested one-way ANOVA P < 0.0001. Sidak’s multiple comparisons test * = P < 0.05 and *** = P < 0.001.

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