Fig. 6: 15q11.2 deletion delays the maturation of synaptic GABA responses. | Translational Psychiatry

Fig. 6: 15q11.2 deletion delays the maturation of synaptic GABA responses.

From: Altered development and network connectivity in a human neuronal model of 15q11.2 deletion-related neurodevelopmental disorders

Fig. 6

A Example raster plots demonstrating acute GABA receptor blockade with application of bicuculline. Raster plots of spikes determined from voltage recordings from 16 electrodes from representative single wells of control (left) and 15q11.2 deletion (right) cultures. Each row is data from a single electrode. Single spikes for a given electrode are represented by black vertical marks. Blue vertical lines are spikes on a single electrode meeting criterion for an electrode burst - 5 spikes occurring within 100 ms. Black tracing at the top of each panel represents spikes per time per second across the entire well. Pink boxes are network bursts. Time scale bar is 40 s. Black vertical line demarcates time of acute bicuculline application. Quantification of bicuculline mediated effects on B weighted mean firing rate (WMFR), C burst percentage and D synchronization in control and deletion cultures. Each data point is the mean activity across 16 electrodes for a single well. Individual symbols are independent cultures with the type of symbol indicating a unique donor. Columns are the mean ± SEM of all cultures with 4 control and 2–3 deletion cultures represented. Two-way ANOVA. Tukey’s multiple comparisons * = P < 0.05, ** = P < 0.01, and **** = P < 0.0001.

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