Fig. 2: Behavioral profiling of Chd8-imKO mice. | Translational Psychiatry

Fig. 2: Behavioral profiling of Chd8-imKO mice.

From: CHD8 adulthood microglial knockdown in C57BL6 mice induces behavioral, morphological, and transcriptional changes in a sex-dependent manner

Fig. 2

Chd8-imKO males: a Time spent in the center of the open field. (*p = 0.0205, t = 2.554, df = 17, two-way student’s t-test) b Time spent in the light area of the dark-light exploration test. (*p = 0.0473, t = 2.149, df = 16, two-way student’s t-test) c Time spent in the open arm of the elevated plus maze (*p = 0.0242, t = 2.527, df = 14, two-way student’s t-test). d Time self-grooming within a 10 min time period. e Number of marbles buried in 30 min time period. f Time spent immobile in tail suspension test (*p = 0.0195, t = 2.580, df = 17, two-way student’s t-test) g Time spent immobile in forced swim test (**p = 0.0037, t = 3.437, df = 15, two-way student’s t-test). h Motor coordination and balance were assessed using the rotarod. Time spent before falling off rotarod. i Percent freezing in each of three tones in cue fear conditioning test (Two way ANOVA, Genotype effect: p = 0.0055, F(1, 17) = 10.09, Tukey’s post hoc test, *p = 0.0172, **p = 0.0016, *p = 0.0173). j Time spent in interaction zone with stranger mice and empty chamber in three-chamber social interaction test (Two way ANOVA, Stranger effect: p < 0.0001, F(1, 62) = 22.68, Tukey’s post hoc test, ***p < 0.0001, nsp = 0.1589). n = wt 9–11 imKO 7–8. *p < 0.05, **p < 0.01, ***p < 0.001. Chd8-imKO females: k Time spent in the center of the open field (*p = 0.0239, t = 2.479, df = 17, two-way student’s t-test). l Time spent in the light area of the dark-light exploration test. m Time spent in the open arm of the elevated plus maze. n Time self-grooming within a 10 min time period. o Number of marbles buried in 30 min time period. p Time spent immobile in tail suspension test q Time spent immobile in forced swim test. r Motor coordination and balance were assessed using the rotarod. Time spent before falling off rotarod. s Percent freezing in each of three tones in cue fear conditioning test t Time spent in interaction zone with stranger mice and empty chamber in three-chamber social interaction test (Two way ANOVA, Stranger effect: p < 0.0001, F(1, 34) = 24.59, Tukey’s post hoc test *p = 0.0169, **p = 0.0027). Further tests determined no differences between experimental groups. (l- dark-light, m- elevated plus maze, p- tail suspension test, q- forced swimming test). n = wt 9–10 imKO 9–10. *p < 0.05, **p < 0.01.

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