Fig. 5: Differential gene expression analysis in Chd8-imKO mice. | Translational Psychiatry

Fig. 5: Differential gene expression analysis in Chd8-imKO mice.

From: CHD8 adulthood microglial knockdown in C57BL6 mice induces behavioral, morphological, and transcriptional changes in a sex-dependent manner

Fig. 5

RNA-Seq analysis was performed on microglia from the whole brain tissues obtained from male and female Chd8-imKO mice. a Microglia sorting from the whole brain and b FACS analysis by three antibodies (CD45+, TMEM119+, Cd11b+) that together identify specifically microglia. The presorted CD45+ population is presented. TMEM119 is represented on the Y axis, and CD11b on the X axis. Blue cells are TMEM+CD11b+, red cells are TMEM-CD11b-, and purple cells are TMEM+CD11b-. c, d Volcano plot illustrating differentially expressed genes in the microglia of c male and d female Chd8-inKO mice. Male microglia displayed 1381 differentially expressed genes (Of those, 773 were upregulated in the imKO microglia and 608 were downregulated) and female microglia displayed 75 differentially expressed genes (Of those, 18 were upregulated in the imKO microglia and 57 were downregulated. e, f Gene ontology of differentially expressed genes in e male and f female imKO mice. Principle Component Analysis showing distribution of all samples in this analysis (n = 4–5 per group) found in Supplementary Fig. 7.

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