Fig. 5: Transcriptomic analysis of prefrontal cortex in Smc3 knockout mice.

At the 6-week mark post-induction of Smc3 knockout through tamoxifen treatment, RNA sequencing was performed from prefrontal cortex (PFC), uncovering differently gene expression profiles. A Volcano plot shows distribution of differently expressed genes of Smc3 cKO male mice compared to WT—87 upregulated genes and 222 downregulated genes. Threshold for differentially expressed genes is FDR < 0.05. B Volcano plot shows distribution of differently expressed genes of Smc3 cKO female mice compared to WT—242 upregulated genes and 616 downregulated genes. C Venn diagram shows overlap and unique sets of downregulated genes identified through RNA sequencing in the prefrontal cortex of male and female mice 6 weeks post-Smc3 knockout induction. D Venn diagram shows overlap and unique sets of upregulated genes identified through RNA sequencing in the PFC of male and female mice. E Gene ontology analysis of differentially expressed genes in males. F Gene ontology analysis of differentially expressed genes in females. G A comparison of differentially expressed genes from our dataset (X axis) and datasets of previous studies that performed RNA sequencing following the knockout of various cohesin components. The graph shows numbers of overlapping downregulated genes from each dataset. Each comparison was analyzed for statistical significance using hypergeometric distribution probability (p-value indicated in parentheses). H A comparison of differentially expressed genes from our dataset (X axis) and datasets of previous studies that performed RNA sequencing following the knockout of various cohesin components. The graph shows numbers of overlapping upregulated genes from each dataset. Each comparison was analyzed for statistical significance using hypergeometric distribution probability (p-value indicated in parentheses).