Fig. 7: Simulated space complex environment affects social behaviors by reducing mPFC^NAc neuronal activity.

a, e Schematic of fiber photometry recording in stage 2 (a) and stage 3 (e) of SDT. b, c, f, g Representative image (b, f) and peri-event plots (c, g) of ΔF/F Ca²⁺ signal changes from mPFC^NAc neuron during sniffing the empty box, familiar mouse and novel mouse in SDT. d, h Comparison of averaged ΔF/F of mPFC^NAc signals in response to SDT during sniffing the empty box, familiar mouse and novel mouse. i, j Heatmap (i) and peri-event plots (j) of Ca²⁺ transients of mPFC^NAc neuron during sniffing the familiar mouse in stage 2 of SDT (CON: n = 5 mice; SSCE: n = 5 mice). l, m Heatmap (l) and peri-event plots (m) of Ca²⁺ transients of mPFC^NAc neuron during sniffing the novel mouse in stage 3 of SDT. k, n The peak Ca²⁺ signal change (ΔF/F) within 5 s during sniffing the familiar mouse in stage 2 (k) and sniffing the novel mouse in stage 3 (n).