Fig. 2: Increased fear memory following conditional knockout of CDYL in dorsal hippocampal excitatory neurons.
From: Activity-dependent phosphorylation of CDYL by CDK5 regulates fear memory in mice
A Schematic of the conditional knockout of CDYL in hippocampal CaMKIIα+ neurons. B Injection site of the virus in the dCA1. Scale bar, 400 μm. C Western blot analysis of CDYL protein levels in the hippocampus. (Unpaired t-test, n = 4 GFP, 4 CRE). D Open field test, time spent in the center area during the first 5 min (left). (Unpaired t test. One outlier excluded, n = 10 WT, 12 cKO). Elevated zero maze, time spent in open arms during the test (right). (Unpaired t test, n = 11 WT, 12 cKO). E Fear conditioning test, contextual fear test (left) and cued fear test (right). The percentage of time spent freezing. (Mann-Whitney test. One outlier excluded, n = 10 GFP, 12 CRE for contextual; n = 11 GFP, 12 CRE for cued).
