Fig. 4: Phosphorylation of CDYL enhances TRIM32-mediated ubiquitination and degradation in response to neural activity.
From: Activity-dependent phosphorylation of CDYL by CDK5 regulates fear memory in mice
A Protein levels of CDYL and phosphorylated CDYL (p-CDYL) in neurons under various treatments. Analyzed by one-way ANOVA from three independent experiments with Tukey’s multiple comparisons test. B Immunoprecipitation analysis of CDYL ubiquitination in treated neurons. CDYL is fused to GFP, and ubiquitin to HA. Lysates were immunoprecipitated with GFP antibody and ubiquitination detected with anti-ubiquitin antibody. C Ubiquitination levels of CDYL in neurons transfected with GFP-tagged CDYL variants: wild-type, CDYL-S147A, or CDYL-S147D, alongside HA-tagged ubiquitin. D Ubiquitination levels of CDYL in neurons treated and transfected as follows: GFP-tagged CDYL, HA-tagged ubiquitin, vector, shTRIM32, or His-tagged TRIM32. Neurons were further treated with KCl in combination with DMSO or roscovitine. All data are represented as mean ± SEM. *p < 0.05, **p < 0.01, ns not significance.
