Fig. 6: Spatial transcriptomic analysis of the brain tissue from Snai2+/+ and Snai2−/− mice. | Translational Psychiatry

Fig. 6: Spatial transcriptomic analysis of the brain tissue from Snai2+/+ and Snai2−/− mice.

From: Developmental arrest of astrocyte lineage in Snai2 deletion mice: implication for the intellectual disability in patients with Waardenburg syndrome

Fig. 6

A UMAP plots of cell clusters in brain tissue of Snai2+/+ and Snai2−/− mice. The UMAP plots on the left panel show 18 cell clusters in brain tissue of Snai2+/+(3833 cells) and Snai2−/− (3899 cells) mice. The stacked bar plots on the right panel show proportion of each cell cluster in brain tissue of Snai2+/+ and Snai2−/− mice. Cell clusters are color-coded as indicated in the legend. B Spatial transcriptomic maps projected onto the same sagittal brain sections from each Snai2+/+ and Snai2−/− mouse. The regions of interest shown here are cerebral cortex, septum, hippocampus, amygdala, thalamus, hypothalamus, and parts of cerebellum and striatum. The mRNA expression profiles relate to 18 cell clusters from C0 to C17, including subtypes of astrocyte, endothelial cell, oligodendrocyte, progenitor cell, and others, in addition to those of neuron. The black dashed lines demarcate the anatomical boundaries of the cerebral cortex and hippocampus, corresponding to clusters C1, C5, C6, and C8 in panel A. C The stacked bar plots show different proportions of certain cell clusters in certain brain regions of Snai2+/+ and Snai2−/− mice. D GSVA of signaling pathways in all (C0-C17) cell clusters of the Snai2−/− mouse brain. E Levels and spatial distribution of c-fos expression in the same sagittal brain sections from each Snai2+/+ and Snai2−/− mouse. The white dashed lines delineate the cerebral cortex and hippocampus, which are known to play pivotal roles in cognitive processes.

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