Fig. 6: Mutations in LCMV CEN active pocket influenced the binding of SAA. | Acta Pharmacologica Sinica

Fig. 6: Mutations in LCMV CEN active pocket influenced the binding of SAA.

From: Salvianolic acid A from Salvia miltiorrhiza identified as a cap-dependent endonuclease inhibitor for pathogenic arenaviruses

Fig. 6

a Analysis of amino acids in LCMV CEN active pocket bond by SAA in docking under diverse conformations; amino acids with binding frequencies equal to or greater than three were taken for mutation analysis. b Affinity of SAA to the LCMV wildtype and mutant CENs. Data were from once representative experiment. c Comparison of the structural formula of SAA and methyl salvionolate A (MSA). d Alignment on the binding conformation of SAA and MSA with LCMV CEN. SAA is shown in green, MSA is shown in blue, and Mn2+ is shown in purple. e Inhibition of MSA on LCMV CEN (left) and LCMV infection (right). SAA was used as a control. Data from enzymatic and cellular assays were obtained from at least three independent experiments.

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