Fig. 2

Changes in TUBB3⁺ nerve distribution over the mouse lifespan. a MIP images of the parietal bone, coronal suture, and frontal bone regions of P0, 4 weeks, 12 weeks, 40 weeks, and 80 weeks mice with TUBB3⁺ nerves. Scale bar is 300 μm. Volume fraction calculation for P0, 4 weeks, 12 weeks, 40 weeks, and 80 weeks TUBB3⁺ nerves in (b) Overall calvaria, (c) Parietal Bone Region, (d) Coronal Suture Region, and (e) Frontal Bone Region, respectively. Data are mean ± SD. Differences were detected using a two-way ANOVA with post-hoc Tukey HSD (Honestly Significant Difference) test. *P < 0.05, **P < 0.01 where designated