Fig. 3

Spatial changes in periosteum and dura mater TUBB3⁺ nerve distribution over the mouse lifespan. a 50 μm coronal cross sections of TUBB3⁺ nerves in P0, 4 weeks, 12 weeks, 40 weeks, and 80 weeks mice. Scale bar is 300 μm. Nerve fraction calculations for TUBB3⁺ periosteal and dural nerves at P0, 4 weeks, 12 weeks, 40 weeks, and 80 weeks in (b) Overall Calvaria, (c) Parietal Bone, (d) Coronal Suture, and (e) Frontal Bone. TUBB3⁺ periosteal nerve volume density calculations at P0, 4 weeks, 12 weeks, 40 weeks, and 80 weeks in (f) Overall Calvaria Periosteum, (g) Parietal Bone Periosteum, (h) Coronal Suture Periosteum, and (i) Frontal Bone Periosteum. TUBB3⁺ dural nerve volume density calculations at P0, 4 weeks, 12 weeks, 40 weeks, and 80 weeks in (j) Overall Calvaria Dura Mater, (k) Parietal Bone Dura Mater, (l) Coronal Suture Dura Mater, (m) Frontal Bone Dura Mater. Data are mean ± SD. Statistics were performed with a two-way ANOVA with post-hoc Tukey HSD test. *P < 0.05, **P < 0.01, ****P < 0.000 1 where designated