Fig. 5
From: 3D imaging reveals changes in the neurovascular architecture of the murine calvarium with aging
Spatial correlation between vessel phenotypes and TUBB3+ nerves. a Masked MIP images of the parietal bone, coronal suture, and frontal bone regions of P0, 4 weeks, 12 weeks, 40 weeks, and 80 weeks mice with TUBB3+ nerves (blue) and vasculature stained with CD31 (green) and Emcn (red). Blood vessel phenotypes have been segmented based on binary masking to visualize CD31hiEmcn-, CD31hiEmcnhi, and CD31loEmcnhi blood vessles. Scale bar is 300 μm. b The fraction of nerves within 10 μm of each vessel phenotype is displayed for (b) P0, (c) 4 weeks, (d) 12 weeks, (e) 40 weeks, and (f) 80 weeks. Data are mean ± SD. Statistics were performed with a two-way ANOVA with post-hoc Tukey HSD test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.000 1 where designated
