Fig. 7

Changes in neurovascular distributions with Apert’s syndrome. a MIP images of the parietal bone, coronal suture, and frontal bone regions of P0 WT and Apert mice with TUBB3⁺ nerves (blue) and vasculature stained with CD31 (green) and Emcn (red). Scale bar is 300 μm. b Overall nerve volume fraction calculation for WT and Apert TUBB3⁺ nerves. c Regional nerve volume fraction calculations in the parietal bone, coronal suture, and frontal bone regions for WT and Apert mice. d Periosteal and dural nerve fraction calculations for TUBB3⁺ nerves in WT and Apert mice. e, f Periosteal and dural nerve volume density calculations for TUBB3⁺ nerves in WT and Apert samples in (e) Overall Calvaria Periosteum, (f) Overall Calvaria Dura Mater. g Total vessel volume fraction calculation for WT and Apert mice. h Phenotypic vessel volume density calculation for CD31^hiEmcn^-, CD31^hiEmcn^hi, and CD31^loEmcn^hi vessels in WT and Apert mice. I Phenotypic vessel phenotype fraction calculation for CD31^hiEmcn^-, CD31^hiEmcn^hi, and CD31^loEmcn^hi vessels in WT and Apert mice. j Spatial association calculations for TUBB3⁺ nerves and vessels phenotypes. The fraction of nerves within 10 μm is displayed for each vessel phenotype