Fig. 1: Evaluation of tissues and Xenium analysis after treatment with anti-PD-L1 combined with chemoradiotherapy. | British Journal of Cancer

Fig. 1: Evaluation of tissues and Xenium analysis after treatment with anti-PD-L1 combined with chemoradiotherapy.

From: Single-cell spatial analysis with Xenium reveals anti-tumour responses of CXCL13 + T and CXCL9+ cells after radiotherapy combined with anti-PD-L1 therapy

Fig. 1

a Characteristics of the tissue samples used in this study. The cell number indicates the number of cells that can be analysed with Xenium. Posttreatment histology revealed stable disease (SD), major pathological response (MPR), and complete response (CR). b Representative HE staining image of pre- and posttreatment tumours. Cancer cells were delineated via HE staining (yellow line). c, d Representative images of HE staining, cell clustering and gene expression. After each cell line was distinguished and utilizing 302 gene expression patterns, pretreatment cells were categorized into 11 clusters, and posttreatment cells were classified into 22 clusters. Each cluster was classified as epithelial (KRT5 + , CDH1 + ), CAF (COL1A1 + ), myeloid (CD68 + , CD163 + ), LYM (lymphocyte, CD3E + , CD4 + , CD8 + ), plasma (POU2AF1 + ), or U.C. (dead cells or unclassified cells).

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