Fig. 1: High Nrf2 expression was associated with disease progression in AML. | Cancer Gene Therapy

Fig. 1: High Nrf2 expression was associated with disease progression in AML.

From: RETRACTED ARTICLE: Nrf2 overexpression increases the resistance of acute myeloid leukemia to cytarabine by inhibiting replication factor C4

Fig. 1

N, normal; CR complete remission; ND, newly diagnosed, RR, relapsed-refractory, P, patient, R, relapse. BR, before relapse. A Protein expression of Nrf2 in normal donors (n = 7) and AML patients (n = 26) was detected by Western blotting. B Relative mRNA expression of Nrf2 in normal (n = 13) and AML patients (n = 50) by RT-qPCR. C Representative images of immunofluorescence of Nrf2 in AML. Scale bars = 20 μm. D Quantification of Nrf2 fluorescence intensity in AML samples by Image J. E Representative images of Immunocytochemistry staining of Nrf2 in AML. Scale bars: 100 and 50 μm from left to right. F, G Nrf2 mRNA and protein expression were detected by RT-qPCR (n = 20) and Western blotting in the same AML patients (n = 15) before and after relapse. Results represented the mean ± SD from 3 separate assays. ***P < 0.001, **P < 0.01, *P < 0.05.

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