Fig. 4: Nrf2 negatively regulated the expression of RFC4. | Cancer Gene Therapy

Fig. 4: Nrf2 negatively regulated the expression of RFC4.

From: RETRACTED ARTICLE: Nrf2 overexpression increases the resistance of acute myeloid leukemia to cytarabine by inhibiting replication factor C4

Fig. 4

EV, empty vector. Ara-C, cytarabine. L-Nrf2, overexpressed Nrf2. siNrf2, silenced Nrf2. A After Nrf2 was overexpressed in AML, expression of RFC4 was detected by Western blotting. B After Nrf2 was silenced in AML, expression of RFC4 was detected by Western blotting. C, D The Nrf2-overexpressing or silencing cells were treated with or without Ara-C (2.5, 1, and 4 μM, respectively) for 24 h. The Nrf2 and RFC4 protein levels were assessed by adopting western blotting. Results represented the mean ± SD from 3 separate assays. ***P < 0.001, **P < 0.01, *P < 0.05.

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