Fig. 5: Nrf2 inhibited RFC4 expression through binding to RFC4 promoter. | Cancer Gene Therapy

Fig. 5: Nrf2 inhibited RFC4 expression through binding to RFC4 promoter.

From: RETRACTED ARTICLE: Nrf2 overexpression increases the resistance of acute myeloid leukemia to cytarabine by inhibiting replication factor C4

Fig. 5

L-Nrf2, overexpressed Nrf2. A Possible Nrf2 binding target sites in RFC4 promoter and diagrams of different luciferase reporter gene expression vectors. B Analysis of DNA enriched fragments of Nrf2 in THP-1 cells by agarose gel electrophoresis. C, D ChIP detected Nrf2 specific sites bind to RFC4 promoter in vivo. Rabbit IgG (negative control) or Nrf2 (positive control) with 10% of the total input chromatin being used as control. E RFC4-promoter wild type and mutant reporter plasmids was cloned into the upstream of luciferase to construct reporter vectors pGL3 and pRL-TK (expressing sea kidney luciferase as an internal reference) as well as its co-transfected with Nrf2 expression vector for 48 h to use double luciferase reporter gene analysis. The luciferase activity of firefly is proportional to the strength of the promoter. Results represented the mean ± SD from 3 separate assays. ***P < 0.001, **P < 0.01, *P < 0.05.

Back to article page