Fig. 8: Overexpression of RFC4 increased the sensitivity of AML cells to Ara-C. | Cancer Gene Therapy

Fig. 8: Overexpression of RFC4 increased the sensitivity of AML cells to Ara-C.

From: RETRACTED ARTICLE: Nrf2 overexpression increases the resistance of acute myeloid leukemia to cytarabine by inhibiting replication factor C4

Fig. 8

EV, empty vector. NC, overexpression plasmid vector (negative control). Ara-C, cytarabine. siNrf2, silenced Nrf2. RFC4, overexpressed RFC4. A–D Overexpressed RFC4 was quantified by RT-qPCR and Western blotting in AML cells. E The apoptosis rates of RFC4-overexpressing AML cells after 24 h of Ara-C treatment (2.5 μM) were measured by flow cytometry. F, G THP-1, U937, and MV4-11 cells were transfected with siNrf2 and targeting RFC4 (RFC4), with EV or NC as control. The expression of RFC4 and Nrf2 were detected by Western blotting. I, J THP-1, U937, and MV4-11 cells were transfected with siNrf2 and targeting RFC4 (RFC4), with EV or NC as control. The cells were treated with Ara-C for 24 h and the apoptosis rates was determined by flow cytometry. Results represented the mean ± SD from 3 separate assays. ***P < 0.001, **P < 0.01, *P < 0.05.

Back to article page