Fig. 7: The dendriplex PUREG4-LA12-anti-GLS1-siRNA is able to cross the new BBB in vitro model with astrocytes. | Cancer Gene Therapy

Fig. 7: The dendriplex PUREG4-LA12-anti-GLS1-siRNA is able to cross the new BBB in vitro model with astrocytes.

From: Lactate-coated polyurea-siRNA dendriplex: a gene therapy-directed and metabolism-based strategy to impair glioblastoma (GBM)

Fig. 7

We established a new in vitro model of BBB with b.End3 cells and astrocytes (iNHA) seeded in each side of a transwell (A). Dendriplexes did not induce iNHA cell death (B). Once the model was established, we exposed it to the dendriplexes, which decreased GLS1 protein expression in both GBM cell lines (C–F), magnification 630×. 1H-NMR analysis of the conditioned media of the basolateral side showed that the dendriplexes crossed the BBB, and induced changes in the exometabolome after 72 h (G, H). All data are represented as mean ± SD. **p < 0.01.

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