Fig. 6: CD40 modulated STING ubiquitination and phosphorylation through interaction with TRAFs.

A, B Detection of STING ubiquitination after CD40 stimulation. C Detection of IRF4 expression, STING phosphorylation, and NF-κB pathway regulation after CD40 stimulation or administration of phosphorylation inhibitors. D Flow cytometry revealed the degrees of B cell differentiation after CD40 stimulation or administration of a phosphorylation inhibitor (APC-CD19 and PE-pre-BCR). E Detection of IRF4 expression, STING phosphorylation, and NF-κB pathway regulation after STING/phosphorylation inhibitor/ubiquitination inhibitor administration. F Flow cytometry revealed the degrees of B cell differentiation after STING/phosphorylation inhibitor/ ubiquitination inhibitor administration (APC-CD19 and PE-pre-BCR).