Fig. 5: scRNA-Seq analysis of CAC-associated macrophages and monocytes in mice with myeloid-specific deletion of Mir34a.
A UMAP plot showing subtypes of macrophages/monocytes from CACs, derived from Mir34aΔMye and Mir34aF/F mice. B Heat-map of top ten differentially expressed genes in each macrophage/monocyte subtype. C UMAP plot showing the Palantir pseudotime of monocytes and macrophages. The color indicates pseudotime directionality from the earliest (blue) to the latest (red). D UMAP plot showing the Monocle analysis of monocytes and macrophages. The color indicates pseudotime directionality from the earliest (blue) to the latest (yellow). E UMAP plot showing the Slingshot analysis of monocytes and macrophages. Terminal cell lineages are indicated. F RNA velocity analysis of monocyte/macrophage subtypes with vector fields representing RNA velocity projected onto the UMAP plot. G Proportions of monocyte/macrophage subtypes in Mir34aΔMye and Mir34aF/F CACs. H Enrichment of indicated MsigDB Hallmark gene sets in differentially expressed genes for each monocyte/macrophage cluster. I Volcano plot of differentially expressed genes between Mir34aΔMye and Mir34aF/F monocyte/macrophage. Predicted and published miR-34a targets are indicated in blue and red, respectively. J Expression of indicated miR-34a target mRNAs in Mir34aΔMye and Mir34aF/F monocytes/macrophages. K UMAP plots of monocytes/macrophages colored by the expression of indicated miR-34a target mRNAs. Significance was determined as described in methods section scRNA-Seq data analysis with p < 0.05*; p < 0.01** and p < 0.001***.
