Fig. 6: Mir34a-deficiency increases migration and polarization towards M2-like macrophages. | Cell Death & Differentiation

Fig. 6: Mir34a-deficiency increases migration and polarization towards M2-like macrophages.

From: Myeloid Mir34a suppresses colitis-associated colon cancer: characterization of mediators by single-cell RNA sequencing

Fig. 6

A Fold change of migrated BMDMs after 4 and 24 h (n = 3). Assay was performed in triplicate inserts. B Percentage of wound closure of CT-26 cells after co-culture with Mir34a-proficient or -deficient BMDMs for 24 h (n = 4). C pri-Mir34a expression in Mir34a-proficient BMDMs after treatment with 0.5 mM NO-donor (n = 3). D pri-miR34a expression after treatment of HCT116 p53 wildtype and p53 knockout cells (n = 3) with a 1 mM NO-donor for 24 h. Expression was normalized to GAPDH. E Mrc1; Arginase-1 and Nos2 expression in Mir34a-proficient and Mir34a-deficient BMDMs (n = 3). F Expression of Mrc1; (G) Arginase-1 and (H) Nos2 in Mir34a-proficient and Mir34a-deficient BMDMs after treatment with IL-4 or LPS + INFγ for 4 h (n = 3). I Immunohistochemical analysis of Arginase-1 (Arg-1) and Nos2 in CACs of Mir34aF/F, Mir34aΔIEC and Mir34aΔMYE mice (n = 3 mice per genotype). Scale bar represent 40 µm. (A–I) Students t-test was used to determine significance. Values represent the mean ± SEM with p < 0.05*; p < 0.01**; p < 0.001*** and p < 0.0001****.

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