Fig. 1: Aux in ECs maintains intestinal homeostasis.

A Compared to control intestines, the number of esg-lacZ+ cells (red, white arrowheads) is dramatically increased in Myo1A^ts>aux^RNAi intestines at 29 °C for 7 days. The white lines indicate the diameter of intestines with indicated genotypes. Quantification of esg-lacZ⁺ cell No/images in different genotypes indicated. Mean ± SD is shown. ****p < 0.0001. Please note that Myo1A^ts>aux^RNAi intestines are highly deformed preventing accurate cell number quantification (the same as follows). B Compared to control intestines, the number of ISCs (by Dl-lacZ, red, white arrowheads) is dramatically increased in Myo1A^ts>aux^RNAi intestines at 29 °C for 7 days. Quantification of Dl-lacZ⁺ cell No/images in different genotypes indicated. Mean ± SD is shown. ****p < 0.0001. C Compared to control intestines, the number of EBs (by Gbe + Su(H)-lacZ, red, white arrowheads) is dramatically increased in Myo1A^ts>aux^RNAi intestines at 29 °C for 7 days. Quantification of Gbe + Su(H)-lacZ⁺ cell No/images in different genotypes indicated. Mean ± SD is shown. ****p < 0.0001. D Compared to control intestines, the number of mitotic cells (white arrowheads) is significantly increased in Myo1A^ts>aux^RNAi intestines at 29 °C for 7 days. Quantification of the number of pH3⁺ cells/images in different genotypes indicated. Mean ± SD is shown. ****p < 0.0001. Scale bars, 20 μm.