Fig. 2: JAK/STAT signaling is ectopically activated upon aux depletion in ECs.

A Compared to control intestines, the number of 10XSTAT-GFP cells and the fluorescence intensity of 10XSTAT-GFP (green, white arrowheads) are significantly increased in Myo1A^ts>aux^RNAi intestines at 29 °C for 7 days. 10XSTAT-GFP channel is showed separately in black white. Quantification of the fluorescence intensity of 10XSTAT-GFP in control and Myo1A^ts>aux^RNAi intestines. Mean ± SD is shown. ****p < 0.0001. B, C Compared to control ECs in which cytokines (by upd-lacZ and upd3-lacZ) are barely detectable, cytokine expression (red, white arrowheads) is significantly increased in aux-defective ECs at 29 °C for 7 days. lacZ channel is showed separately in black white. Quantification of the fluorescence intensity of Upds-lacZ in control and Myo1A^ts>aux^RNAi ECs. Mean ± SD is shown. ****p < 0.0001. D The transcripts of cytokines and socs36E are significantly increased in Myo1A^ts>aux^RNAi intestines. Mean ± SD is shown. ****p < 0.0001. E The dramatic increase of Dl⁺ cells (red, white arrowheads) in Myo1A^ts>aux^RNAi intestines is significantly suppressed by further removal of upd3. Quantification of the number of Dl⁺ cells in intestines with indicated genotypes. Mean ± SD is shown. ****p < 0.0001. Scale bars: 20 μm.