Fig. 4: COPII vesicles are altered upon aux depletion in ECs.

A Compared to control intestines, the fluorescence intensity of KDEL-GFP (green, white arrowheads) is significantly increased in Myo1A^ts>aux^RNAi intestines at 29 °C for 7 days. KDEL-GFP channel is showed separately in black white. Quantification of the fluorescence intensity of KDEL-GFP in control and Myo1A^ts>aux^RNAi intestines. Mean ± SD is shown. ****p < 0.0001. B Compared to control intestines, the fluorescence intensity of Ergic53-GFP (green, white arrowheads) is significantly increased in Myo1A^ts>aux^RNAi intestines at 29 °C for 7 days. Ergic53-GFP channel is showed separately in black white. Quantification of the fluorescence intensity of Ergic53-GFP in control and Myo1A^ts>aux^RNAi intestines. Mean ± SD is shown. ****p < 0.0001. C Compared to control intestines, the number of Sec13-RFP⁺ puncta (red, white arrowheads) is significantly increased in Myo1A^ts>aux^RNAi intestines at 29 °C for 7 days. Sec13-RFP channel is showed separately in black white. Quantification of the size of Sec13-RFP⁺ puncta and the number of Sec13-RFP⁺ puncta/EC in control and Myo1A^ts>aux^RNAi intestines. Mean ± SD is shown. ****p < 0.0001. D Compared to control intestines, the number of Sec31-RFP⁺ puncta (red, white arrowheads) is significantly increased in Myo1A^ts>aux^RNAi intestines at 29 °C for 7 days. Sec31-RFP channel is showed separately in black white. Quantification of the size of Sec31-RFP⁺ puncta and the number of Sec31-RFP⁺ puncta/EC in control and Myo1A^ts>aux^RNAi intestines. Mean ± SD is shown. ****p < 0.0001. Scale bars, 5 μm (D, 10 μm).