Fig. 7: CAMs in ECs are required for intestinal homeostasis.
From: Auxilin in enterocytes controls intestinal homeostasis through inter-cell communication
A Compared to control, the number of progenitors (by esg-lacZ, red, white arrowheads) is dramatically increased when shg, mew, and if are depleted in ECs at 29 °C for 7 days, while no obvious changes are observed upon arm knockdown. Quantification of esg-lacZ+ cell No/images in different genotypes indicated. Mean ± SD is shown. ****p < 0.0001. B Model of Aux in ECs facilitating COPII-mediated ER-to-GA trafficking of CAMs to control intestinal homeostasis through cell-cell communication. Under physiological conditions, Aux facilitates COPII-mediated transport of CAMs to stabilize EC integrity, thereby maintaining intestinal homeostasis. Whilst in aux-depleted ECs, the COPII-mediated transport of CAMs is blocked. aux-depleted ECs undergo apoptosis due to extended ER stress and secrete cytokines to activate JAK/STAT signaling in ISCs, eventually leading to intestinal homeostasis disruption and pathogenesis. Scale bars, 20 μm.
