Fig. 1: Highly expressed Tet2 in normal kidney and renal epithelial tubular cells.
From: DNA demethylase Tet2 suppresses cisplatin-induced acute kidney injury
A Immunofluorescence in HK-2 cells showing the location of Tet2 (green, orange arrows) predominantly in the nucleus (DAPI, blue), while the signal of Tet2 was attenuated by RNAi. Scale bar, 15 μm. B, C Both in vivo and in vitro qPCR analyses showing high expression of Tet2. ***P < 0.001 versus Tet1 gene; +++P < 0.001 versus Tet3 gene (n = 3). D Male C57BL/6 mice intraperitoneally injected with 0.9% saline or 22 mg/kg cisplatin (n = 6). Immunohistochemistry revealed a reduced Tet2 expression in the AKI model (original magnification, ×200). E, F HK-2 cells were treated with 10 µM cisplatin to mimic acute injury. E qPCR analyses showing a significant downregulation of Tet2 mRNA in the cisplatin-treated HK-2 cells compared to control cells. F Western blot analyses demonstrate a decreased Tet2 protein in the HK-2 cells after cisplatin treatment compared to the control cells.
