Fig. 2: Characterization of Tet2-KO mice.
From: DNA demethylase Tet2 suppresses cisplatin-induced acute kidney injury
A The schematic diagram showing the crossbreeding strategy of Tet2-floxed mice (Tet2f/f) with EIIa-Cre transgenic mice. B Mice genotyping by PCR analyses of genomic DNA. Lane 1: Tet2f/f, Cre+/−, designated as KO, Lane 2: Tet2f/f, Cre−/−, designated as WT. C Real-time PCR analyses demonstrated a substantial reduction of the spleen and renal Tet2 mRNA in KO mice compared to WT mice. ***P < 0.001 versus WT mice. E–H Tet2 gene deletion had no impact on phenotype. There was no significant difference in the body weight (E), kidney weight (F), Scr (G), BUN (H) between KO and WT mice at 2 and 4 months (n = 6).
