Fig. 3: Tet2 KO accelerates AKI progression after cisplatin injection.

A, B Cispaltin treatment (22 mg/kg) led to a marked elevation of both Scr and BUN in KO + CIS mice compared to the KO and WT + CIS mice (n = 6). C Representative images of HE-stained kidney sections (200×). D Histologic damage in HE-stained kidney sections (n = 6) was scored by the criterion used in previous research. Ten randomly selected fields (original magnification, ×200) per kidney were required for counting the percentage of tubules that displayed cast formation, tubular necrosis, and dilation as follows: 0 = normal; 1 ≤ 10%; 2 = 10–25%; 3 = 26–50%; 4 = 51–75%; 5 ≥ 75%. E, F qRT-PCR analyses revealed a remarkable increase in KIM-1 and NGAL expression in KO + CIS mice, compared to KO and WT + CIS mice. *P < 0.05, **P < 0.01, and ***P < 0.001 represent statistical significance between WT + CIS mice or KO + CIS mice versus KO mice; ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001 represent statistical significance between WT + CIS mice or KO + CIS mice versus WT mice; ⁺P < 0.05, ⁺⁺P < 0.01, and ⁺⁺⁺P < 0.001 represent statistical significance between KO + CIS mice versus WT + CIS mice.