Fig. 4: 8-Br-cGMP induces apoptosis by phosphorylating HSPB6.
From: 8-Br-cGMP activates HSPB6 and increases the antineoplastic activity of quinidine in prostate cancer
A Differentially expressed genes between HSPB6-High PRAD and HSPB6-Low PRAD from TCGA database. B The results of the KEGG analysis of the differential genes. C The image represented the results of the GSEA analysis of the differential genes. D Western blot analysis showed that the expression of p-HSPB6, p-VASP, and Cleaved-Caspase-3 after knockdown HSPB6 and (or) supplementation with 8-Br-cGMP (1 mM) (p-VASP as positive control). E, F Colony formation assays (E) and CCK8 (F) showed the proliferation ability of DU145 and C4-2 after knockdown HSPB6 and (or) supplementation with 8-Br-cGMP (1 mM). (P < 0.05 as “*“, P < 0.01 as “**“, P < 0.001 as “***“, P < 0.0001 as “****“, unpaired t-test, ANOVA.) G Flow cytometry showed the apoptosis levels of DU145 and C4-2 after knockdown HSPB6 and (or) supplementation with 8-Br-cGMP (1 mM). (P < 0.05 as “*“, P < 0.01 as “**“, P < 0.001 as “***“, P < 0.0001 as “****“, unpaired t test).
