Fig. 5: p-HSPB6 replaces p-Cofilin from YWHAG to induce apoptosis.
From: 8-Br-cGMP activates HSPB6 and increases the antineoplastic activity of quinidine in prostate cancer
A Subcellular localization of HSPB6, Cofilin, and YWHAG from the GeneCards database. B IF staining results of HSPB6, Cofilin, and YWHAG in different cells from the HPA database. C IF staining results of HSPB6, Cofilin, and YWHAG in prostate cancer. D Co-IP results showed the binding of YWHAG to p-HSPB6 and p-Cofilin with or without overexpression of HSPB6. E Western blot analysis showed that the expression of HSPB6, p-HSPB6, Cofilin, and p-Cofilin changed after overexpression of HSPB6. F The mechanism by which p-HSPB6 replaces p-Cofilin from YWHAG. G Colony formation assays showed the proliferation ability of DU145 and C4-2 after the knockdown of Cofilin and (or) overexpression of HSPB6. H Flow cytometry showed the apoptosis levels of DU145 and C4-2 after the knockdown of Cofilin and (or) overexpression of HSPB6. (P < 0.05 as “*“, P < 0.001 as “***“, P < 0.0001 as “****“, unpaired t-test).
