Fig. 7: E2F1 regulates the transcription of HSPB6 and PROSER3.
From: 8-Br-cGMP activates HSPB6 and increases the antineoplastic activity of quinidine in prostate cancer
A, B RT-qPCR (A) and Western blot analysis (B) showed the changes in HSPB6 and PROSER3 expression after knockdown E2F1. C, D RT-qPCR (C) and Western blot analysis (D) showed the changes in HSPB6 and PROSER3 expression after overexpression of E2F1. E Location of HSPB6 and PROSER3 promoter on the chromosome. F Fluorescent plasmids constructed by different truncations of the HSPB6 and PROSER3 promoter. G The image represented the fluorescence intensity after co-transfection of different fluorescent plasmids and E2F1 plasmids. H CCK8 showed the proliferation ability of DU145 and C4-2 after overexpression of E2F1 and HSPB6. (P < 0.05 as “*”, P < 0.001 as “***”, P < 0.0001 as “****”, ANOVA.) I Western blot analysis showed that the expression of the apoptosis marker Cleaved-Caspase-3 changed after overexpression of E2F1 and HSPB6.
