Fig. 6: DDX3 regulates the protein expression of candidate target genes involved in oogenesis in HeLa cells.
From: DDX3 is critical for female fertility via translational control in oogenesis
HeLa cells were transduced with the empty lentiviral vector (pLKO.1) or the pLKO.1 vector expressing DDX3 shRNAs (shDDX3-1 and shDDX3-2). Cells were harvested for analysis at day 3 post-transduction. A Western blot analysis was performed using antibodies against DDX3, APC1, cyclin E1, p38 MAPK, B56β, PP2Aβ, PKACα, FBXW11, Rac1, GNAS, and α-tubulin proteins. Detection of α-tubulin served as a loading control. B The expression of APC1, CCNE1, P38γ, B56β, PP2Aβ, PKACα, FBXW11, RAC1, P38β, GNAS, DDX3, and GAPDH mRNAs were detected by quantitative real-time RT-PCR. The bar graph shows the relative mRNA levels normalized to GAPDH as mean and standard deviation from three independent experiments. Statistical significance was tested by one-way ANOVA (ns not significant; **p < 0.01; ***p < 0.001; ****p < 0.0001).
