Fig. 1: Establishment and confirmation of a novel cardiomyocyte-specific MIAT conditional knockout mouse line. | Cell Death Discovery

Fig. 1: Establishment and confirmation of a novel cardiomyocyte-specific MIAT conditional knockout mouse line.

From: Cardiomyocyte-restricted MIAT deletion is sufficient to protect against murine myocardial infarction

Fig. 1

A Targeting scheme, mouse crossing, and establishment of cardiomyocyte (CM)-restricted conditional knockout (cKO) of MIAT in vivo. Top, Targeting scheme and mouse crossing. Bottom, Representative genotyping results of MIATfl/fl and αMHC-Cre mice. Target (tg) and control (ctrl) bands are shown. Neg.= negative control and Pos.= positive control. B–D QRT-PCR analyses of MIAT (B), miR-150-5p (a known direct target of MIAT; C), and Sprr1a (a known direct target of miR-150-5p; D) in left ventricles from adult MIATfl/fl or MIAT cKO (i.e., MIATfl/fl;αMHC-Cre) mice. N = 3–4 per group. Data are presented as mean ± SEM. Unpaired 2-tailed t-test. *P < 0.05 or **P < 0.01 vs. MIATfl/fl mice.

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