Log in or create a free account to read this content
Gain free access to this article, as well as selected content from this journal and more on nature.com
or
References
Coutu, D. L., Kokkaliaris, K. D., Kunz, L. & Schroeder, T. Nat. Biotechnol. 35, 1202–1210 (2017).
Acar, M. et al. Nature 526, 126–130 (2015).
Chung, K. et al. Nature 497, 332–337 (2013).
Renier, N. et al. Cell 165, 1789–1802 (2016).
Pan, C. et al. Nat. Methods 13, 859–867 (2016).
Susaki, E. A. et al. Cell 157, 726–739 (2014).
Greenbaum, A. et al. Sci. Transl. Med. 9, eaah6518 (2017).
Louveau, A. et al. Nature 523, 337–341 (2015).
Lucas, D. et al. Nat. Med. 19, 695–703 (2013).
Decosterd, I. & Woolf, C. J. Pain 87, 149–158 (2000).
Osterloh, J. M. et al. Science 337, 481–484 (2012).
Yang, J. et al. Cell 160, 161–176 (2015).
Geisler, S. et al. Brain 139, 3092–3108 (2016).
Turkiew, E., Falconer, D., Reed, N. & Hoke, A. J. Peripher. Nerv. Syst. 22, 162–171 (2017).
Acknowledgements
This study was supported by the State Key Laboratory of Membrane Biology, the IDG/McGovern Institute for Brain Research, the Center for Life Sciences and the School of Life Sciences at Peking University, and by the National Natural Science Foundation of China (31522024 and 31771111).
Author information
Authors and Affiliations
Contributions
J.Y. conceived and designed the project as the senior author. Q.W., K.L., L.Y. and H.W. performed the experiments and analyzed the results under the supervision of J.Y. J.Y. wrote the manuscript with the assistance of Q.W., K.L., L.Y. and H.W.
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing interests.
Rights and permissions
About this article
Cite this article
Wang, Q., Liu, K., Yang, L. et al. BoneClear: whole-tissue immunolabeling of the intact mouse bones for 3D imaging of neural anatomy and pathology. Cell Res 29, 870–872 (2019). https://doi.org/10.1038/s41422-019-0217-9
Received:
Accepted:
Published:
Issue date:
DOI: https://doi.org/10.1038/s41422-019-0217-9
This article is cited by
-
Divide-and-conquer strategy with engineered ossification center organoids for rapid bone healing through developmental cell recruitment
Nature Communications (2025)
-
Deep imaging of LepR+ stromal cells in optically cleared murine bone hemisections
Bone Research (2025)
-
Schnurri-3 inhibition rescues skeletal fragility and vascular skeletal stem cell niche pathology in the OIM model of osteogenesis imperfecta
Bone Research (2024)
-
MarShie: a clearing protocol for 3D analysis of single cells throughout the bone marrow at subcellular resolution
Nature Communications (2024)
-
Multiple cancer cell types release LIF and Gal3 to hijack neural signals
Cell Research (2024)
