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Data availability
The corresponding coordinates and cryo-EM density map have been deposited in the Protein Data Bank (http://www.rcsb.org/pdb) with code 7EXD, and in EMDB (http://www.ebi.ac.uk/pdbe/emdb/) with code EMD-31371.
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Acknowledgements
The cryo-EM data were collected at the Cryo-Electron Microscopy Research Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences (Shanghai, China). This work was partially supported by the Ministry of Science and Technology of China (2018YFA0507002 to HEX), Strategic Priority Research Program of the Chinese Academy of Sciences (XDB37030103 to HEX), Shanghai Municipal Science and Technology Major Project (2019SHZDZX02 to HEX), the National Natural Science Foundation of China (31770796 to YJ), National Science & Technology Major Project “Key New Drug Creation and Manufacturing Program” (2018ZX09711002-002-002 to YJ).
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SH and PX designed the expression constructs, purified the complexes, prepared samples for negative stain and data collection toward the structures, performed functional assay, prepared the figures and manuscript draft. PX evaluated the specimen by negative-stain EM, screened the cryo-EM conditions, prepared the cryo-EM grids, collected cryo-EM images, built the model, and refined the structures. YT, CY, and YZ participated in the NanoBiT G protein recruitment assays. YJ participated in the supervision of SH, PX, YT, CY and YZ, analyzed the structures, and edited the manuscript. HEX conceived and supervised the project, analyzed the structures, and wrote the manuscript with inputs from all authors.
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Huang, S., Xu, P., Tan, Y. et al. Structural basis for recognition of anti-migraine drug lasmiditan by the serotonin receptor 5-HT1F–G protein complex. Cell Res 31, 1036–1038 (2021). https://doi.org/10.1038/s41422-021-00527-4
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DOI: https://doi.org/10.1038/s41422-021-00527-4
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